RBS
Part:BBa_K2355219:Design
Designed by: Philip Srensen Group: iGEM17_DTU-Denmark (2017-10-27)
Strong promoter with RFC[25] scars and a startcodon
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We has to make this part, with added RFC[25] scars, as the composite part system on the registry doesn't allow us to add our own sequences to a composite part, and because the registry system automatically assumes that the RFC[10] standard was used for 3A assembly, even though the RFC[10] will create uneven scars, thereby introducing a frame-shift in any fusion protein assembled this way.
Source
Part BBa_B0030 was used as the RBS in this part